Enzyme-free genetic copying of DNA and RNA sequences

• Marilyne Sosson and
• Clemens Richert

Beilstein J. Org. Chem. 2018, 14, 603–617, doi:10.3762/bjoc.14.47

• were chosen because a large body of literature exists on their reactions including studies by Orgel [35], Kanavarioti [31][36], Szostak [37][38], and Göbel [39]. The oxyazabenzotriazolides are our preferred monomers because they gave us the fastest primer extensions, both on RNA and on DNA templates

Grip on complexity in chemical reaction networks

• Albert S. Y. Wong and
• Wilhelm T. S. Huck

Beilstein J. Org. Chem. 2017, 13, 1486–1497, doi:10.3762/bjoc.13.147

• networks exhibiting oscillatory behavior. Within this framework, Figure 4b shows an in vitro implementation of an oscillator comprising a positive and a delayed negative feedback loop [90]. The canonical gene regulation pathway uses the information encoded in DNA templates T1−T3. Similarly, a genetic

DNA functionalization by dynamic chemistry

• Zeynep Kanlidere,
• Oleg Jochim,
• Marta Cal and
• Ulf Diederichsen

Beilstein J. Org. Chem. 2016, 12, 2136–2144, doi:10.3762/bjoc.12.203

• oligonucleotides. The amino group containing phosphoramidite was used together with complementary single-strand DNA templates that influenced the Watson–Crick base-pairing equilibrium in the mixture with a set of aldehyde modified nucleobases. A significant fraction of all possible base-pair mismatches was

• analogue. Keywords: base-pairing; base-pair mismatch; DNA functionalization; DNA templates; dynamic combinatorial chemistry; D-threoninol based scaffolds; Introduction The well-defined duplex structure, self-assembling by base-pair recognition, and the accessibility by solid-phase synthesis make DNA

• . Here we synthesized the phosphoramidite building blocks derived from D-threoninol which contain protected amine or thiol groups. These building blocks are used for later incorporation into oligonucleotides via solid-phase synthesis. Using these modified oligonucleotides and single strand DNA templates

DNA display of glycoconjugates to emulate oligomeric interactions of glycans

• Alexandre Novoa and
• Nicolas Winssinger

Beilstein J. Org. Chem. 2015, 11, 707–719, doi:10.3762/bjoc.11.81

• different chemistries used in the glycoconjugations and the different strategies used to display the glycans with DNA templates. Review Glycan–DNA conjugates An initial solution used in the pioneering work of Kobayashi [17] for nucleic acid–glycan conjugation was the chemoselective reaction of p

• in the glycans. DC-SIGN is a tetrameric lectin implicated in interactions with a broad array of pathogens including HIV. A library of 37,485 assemblies was prepared by hybridization of two sets of PNA-tagged glycoconjugates onto a library of DNA templates (Figure 4). Screening the library by affinity

• set of five different glycan–PNA conjugates and different DNA templates, the optimal spatial arrangement of the ligands was systematically investigated. Additionally, the flexibility of the PNA–DNA duplexes was also modulated by introducing nick-sites and partially unpaired regions in the DNA display